Application

Anti-BRCA1 Antibody, clone BR64 is a high quality Mouse Monoclonal Antibody for the detection of BRCA1 & has been validated in IP & WB.

Immunoprecipitation

Western blot

Optimal working dilutions must be determined by end user.

Immunogen

Purified polypeptide representing a 100-amino acid segment of the amino terminus of the BRCA1 gene product.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Format: Purified

Quality

Tested

Specificity

The breast cancer susceptibility gene, BRCA1*, is located on chromosome 17 (at q21), and is mutated in the majority of heritable breast cancers. The mutations within the coding region occur throughout the gene with little apparent clustering. The majority of BRCA1 mutations result in shortened BRCA1 gene products, creating a need for an amino-terminal marker. Antibody MAB4132 recognizes both the full-length and mutated (shortened) forms of BRCA1 in breast cancer cell lines. It also recognizes over-expressed protein in transiently transfected Cos cells. A single cross-reacting protein of approximately 27 kDa is also seen upon immunoprecipitation. No other cross-reacting proteins are detected.

BRAC1 is 220kDa.

*BRCA1 is covered under U.S. patents 5,747,282, 5,753,441 and 6,162,897.